Talk:Macromolecular crowding

A fact from Macromolecular crowding appeared on Wikipedia's Main Page in the Did you know column on 15 September 2008, and was viewed approximately 2,631 times (disclaimer) (check views). The text of the entry was as follows:

Did you know... that macromolecular crowding (pictured) can make molecules in cells behave in radically different ways than in test-tube enzyme assays?

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New section that does not fit well in article[edit]

Can we use this somehow? Reads like a journal article Tim Vickers (talk) 14:59, 17 May 2024 (UTC)[reply]

Macromolecular crowding in regenerative medicine[edit]

Satyam et al. from National University of Ireland, Galway (NUI Galway) proposed macromolecular crowding as means to create ECM-rich tissue equivalents. The principle of macromolecular crowding is derived from the notion that in vivo cells reside in a highly crowded/dense extracellular space and therefore the conversion of the de novo synthesised procollagen to collagen I is rapid. However, in the even substantially more dilute than body fluids (e.g., urine: 36–50 g/L; blood: 80 g/L) culture conditions (e.g., HAM F10 nutrient medium: 16.55 g/L; DMEM/ F12 medium: 16.78 g/L; DMEM high glucose and L-glutamine medium: 17.22 g/L), the rate limiting conversion of procollagen to collagen I is very slow. It was confirmed that the addition of inert polydispersed macromolecules (presented as spherical objects of variable diameter) in the culture media will facilitate amplified production of ECM-rich living substitutes. Macromolecular crowding, by imitating native tissue localised density, can be utilised to effectively modulate in vitro microenvironments and ultimately produce ECM-rich cell substitutes, within hours rather than days or months in culture, without compromising fundamental cellular functions.^[1]^[2]^[3]^[4]

^ Satyam, Abhigyan; Kumar, Pramod; Fan, Xingliang; Gorelov, Alexander; Rochev, Yury; Joshi, Lokesh; Peinado, Héctor; Lyden, David; Thomas, Benjamin (2014-05-21). "Macromolecular crowding meets tissue engineering by self-assembly: a paradigm shift in regenerative medicine". Advanced Materials. 26 (19): 3024–3034. Bibcode:2014AdM....26.3024S. doi:10.1002/adma.201304428. hdl:10379/15414. ISSN 1521-4095. PMID 24505025. S2CID 31522448.
^ Kumar, Pramod; Satyam, Abhigyan; Fan, Xingliang; Collin, Estelle; Rochev, Yury; Rodriguez, Brian J.; Gorelov, Alexander; Dillon, Simon; Joshi, Lokesh (2015-03-04). "Macromolecularly crowded in vitro microenvironments accelerate the production of extracellular matrix-rich supramolecular assemblies". Scientific Reports. 5: 8729. Bibcode:2015NatSR...5E8729K. doi:10.1038/srep08729. ISSN 2045-2322. PMC 4348624. PMID 25736020.
^ Satyam, Abhigyan; Kumar, Pramod; Cigognini, Daniela; Pandit, Abhay; Zeugolis, Dimitrios I. (2016-10-15). "Low, but not too low, oxygen tension and macromolecular crowding accelerate extracellular matrix deposition in human dermal fibroblast culture". Acta Biomaterialia. 44: 221–231. doi:10.1016/j.actbio.2016.08.008. ISSN 1878-7568. PMID 27506127.
^ EP 2532736, Zeugolis, Dimitrios & Satyam, Abhigyan, "Engineered living tissue substitute", published Dec 12, 2012

[1] Satyam, Abhigyan; Kumar, Pramod; Fan, Xingliang; Gorelov, Alexander; Rochev, Yury; Joshi, Lokesh; Peinado, Héctor; Lyden, David; Thomas, Benjamin (2014-05-21). "Macromolecular crowding meets tissue engineering by self-assembly: a paradigm shift in regenerative medicine". Advanced Materials. 26 (19): 3024–3034. Bibcode:2014AdM....26.3024S. doi:10.1002/adma.201304428. hdl:10379/15414. ISSN 1521-4095. PMID 24505025. S2CID 31522448.

[2] Kumar, Pramod; Satyam, Abhigyan; Fan, Xingliang; Collin, Estelle; Rochev, Yury; Rodriguez, Brian J.; Gorelov, Alexander; Dillon, Simon; Joshi, Lokesh (2015-03-04). "Macromolecularly crowded in vitro microenvironments accelerate the production of extracellular matrix-rich supramolecular assemblies". Scientific Reports. 5: 8729. Bibcode:2015NatSR...5E8729K. doi:10.1038/srep08729. ISSN 2045-2322. PMC 4348624. PMID 25736020.

[3] Satyam, Abhigyan; Kumar, Pramod; Cigognini, Daniela; Pandit, Abhay; Zeugolis, Dimitrios I. (2016-10-15). "Low, but not too low, oxygen tension and macromolecular crowding accelerate extracellular matrix deposition in human dermal fibroblast culture". Acta Biomaterialia. 44: 221–231. doi:10.1016/j.actbio.2016.08.008. ISSN 1878-7568. PMID 27506127.

[4] EP 2532736, Zeugolis, Dimitrios & Satyam, Abhigyan, "Engineered living tissue substitute", published Dec 12, 2012

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